Manual determination of the saccharification of lignin deficient tobacco. Samples from wild type, a double mutant for cinnamyl alcohol dehydrogenase (CAD) and caffeate O-methyltransferase (COMT) (cadcomt), double cinnamoyl-CoA reductase (CCR) and COMT material (ccrcomt) and a CCR mutant line (ccr) were compared after 8 hours of hydrolysis. The samples were heated in the presence of 0.5N NaOH, and subsequently rinsed before the enzymatic digestion. The experiment was carried out in triplicate. Bars represent values ± SD.