Time course of the saccharification of lignin deficient tobacco material performed in the automated platform. Samples from wild type, a double mutant for cinnamyl alcohol dehydrogenase (CAD) and caffeate O-methyltransferase (COMT) (cadcomt), double cinnamoyl-CoA reductase (CCR) and COMT material (ccrcomt) and a CCR mutant line (ccr) were loaded into 96-well plates and analysed using the high throughput platform. Sample aliquots from the hydrolytes were taken and assayed for reducing sugar equivalents after 2, 6, 12 and 18 hours. Results are representative of experiments performed in triplicate. Bars represent values ± SD of eight experiments.