Biodetoxification of toxins generated from lignocellulose pretreatment using a newly isolated fungus, Amorphotheca resinae ZN1, and the consequent ethanol fermentation

Table 9 Comparisons of the two detoxification methods for pretreated CS^a

	Glucose	Xylose	Acetic acid	Levulinic acid	HMF	Furfural	DM loss	Ethanol yield	Water usage
	(mg/g DM)						(%)	(%, wt/wt)	(kg/kg DM)
Original	10.7 ± 0.2	52.6 ± 2.1	12.3 ± 0.1	2.2 ± 0.2	4.1 ± 0.3	6.2 ± 0.5	0.0	10.3	0.0
Biodetoxified	4.0 ± 0.4	22.1 ± 0.8	0.1 ± 0.1	0.6 ± 0.2	3.4 ± 0.2	0.0 ± 0.1	0.0	56.5	0.0
water washing
L/S ratio 1:1	3.1 ± 0.2	15.3 ± 1.3	3.3 ± 0.0	0.6 ± 0.2	0.8 ± 0.2	1.1 ± 0.1	18.5 ± 0.8	47.4	2.0 ± 0.3
L/S ratio 3:1	2.0 ± 0.0	7.6 ± 0.5	2.1 ± 0.2	0.5 ± 0.3	0.7 ± 0.1	0.9 ± 0.2	21.3 ± 0.6	49.3	6.0 ± 0.6
L/S ratio 5:1	1.2 ± 0.1	6.1 ± 0.0	1.4 ± 0.3	0.2 ± 0.1	0.4 ± 0.1	0.8 ± 0.2	27.2 ± 1.2	45.9	10.0 ± 1.0

^aL/S ratio indicates the freshwater usage vs. the weight of the pretreated CS (wt/wt) during the washing step. Conditions for biodetoxification: 25°C for 4 days in a static incubator. Conditions for SSF: 30% (wt/wt) solid loading, 15.0 FPU/g DM, pH 5.0, in the helical stirring bioreactor at 150 rpm. FPU, unit of filter paper cellulase.

ISSN: 2731-3654