Table 1 Plasmids and strains used in the current study
From: Comparison of heterologous xylose transporters in recombinant Saccharomyces cerevisiae
Strains and plasmids | Relevant genotype | Reference |
|---|---|---|
Plasmids | ||
YIplac128 | LEU2 | [33] |
YIpOB9 | URA3 TDH3p-XYL1(K270R)-ADH1t, PGK1p-XYL2-PGK1t | [19] |
pSUT1 | Current study | |
pAt5g5920 | Current study | |
YIpDR1 | YIplac128 TDH3p-GXF1-CYC1t | [9] |
YIpDR4 | YIplac128 TDH3p-SUT1-CYC1t | Current study |
YIpDR5 | YIplac128 TDH3p-At5g5920-CYC1t | Current study |
S. cerevisiae strains | ||
TMB 3043 | CEN.PK 2-1C Δgre3, his3::PGK1 p-XKS1-PGK1 t, TAL1::PGK1 p-TAL1-PGK1 t, TKL1::PGK1 p-TKL1-PGK1 t, RKI 1::PGK1 p-RKI1-PGK1 t, RPE1::PGK1 p-RPE1-PGK1 t, leu2, ura3 | [34] |
TMB 3662 | TMB 3043, ura3::YIpOB9 | [19] |
TMB 3415 | TMB 3662, leu2::YIplac128 | Current study |
TMB 3416 | TMB 3662, leu2::YIpDR1 | Current study |
TMB 3418 | TMB 3662, leu2::YIpDR4 | Current study |
TMB 3419 | TMB 3662, leu2::YIpDR5 | Current study |