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Table 1 Comparison of GUSPlus expression in switchgrass seedlings induced by four Agrobacterium strains, at different acetosyringone concentrations.

From: A high-throughput transient gene expression system for switchgrass (Panicum virgatum L.) seedlings

Agrobacterium strains

Acetosyringone, μM

GUS-positive seedlings

 

0

50

100

200

Mean ± SD*

Total, n†

%

AGL1

2

6

14

14

9.0 ± 3.0a

36

6.0

EHA105

2

4

7

7

5.0 ± 1.2b

22

3.7

GV3101

2

2

2

1

1.5 ± 0.3c

7

1.2

C58

1

1

0

0

0.5 ± 0.3c

2

0.1

  1. AS = acetosyringone; GUS = β-glucuronidase.
  2. *Means followed by the same letter (a, b, c) were not significantly different at the 5% level using The Tukey multiple comparison test.
  3. † Three replicates of 50 seedlings tested with each strain at each acetosyringone concentration.