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Table 2 A comparison of thiol compounds and their effect on transient gene expression of GUSPlus.

From: A high-throughput transient gene expression system for switchgrass (Panicum virgatum L.) seedlings

Treatment*

GUS-positive seedlings

 

Total, n*

Mean ± SE†

%

H2O, AS

21

7.0 ± 0.6b

14.0

H2O, cysteine, AS

26

8.7 ± 2.3b

17.3

H2O, DTT, AS

29

9.7 ± 2.2b

19.3

H2O, cysteine, DTT, AS

47

15.7 ± 1.9a

31.3

  1. AS = acetosyringone; DTT = dithiothreitol.
  2. All of the treatments included 1 minute of sonication.
  3. *Three replicates of 50 seedlings per replicate were tested in each treatment.
  4. †Means followed by the same letter (a, b, c) were not significantly different at the 5% level as determined by The Tukey multiple comparison test.