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Table 4 A comparison of vacuum infiltration and separation by centrifugation on GUSPlus expression in sonicated switchgrass seedlings.

From: A high-throughput transient gene expression system for switchgrass (Panicum virgatum L.) seedlings

Treatment* GUS-positive seedlings
  Total, n* Mean ± SE† %
Control-1 (no vacuum; no separation by centrifugation) 17 4.2 ± 0.6b 8.5
Control-2 (no vacuum; separation by centrifugation) 43 10.8 ± 2.6a 21.5
Vacuum (1 minute); separation by centrifugation 43 10.8 ± 1.6a 21.5
Vacuum (2 minutes); separation by centrifugation 44 11.0 ± 2.1a 22.0
Vacuum (4 minutes); separation by centrifugation 49 12.2 ± 3.2a 24.5
Vacuum (8 minutes); separation by centrifugation 50 12.5 ± 0.5a 25.0
Vacuum (16 minutes); separation by centrifugation 55 13.8 ± 1.4a 27.5
  1. + All seedlings were sonicated for 1 minute.
  2. *Three replicates of 50 seedlings per replicate were tested in each treatment.
  3. †Means followed by the same letter (a, b, c) were not significantly different at the 5% level as determined by The Tukey multiple comparison test.