Figure 6
High-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) of hydrolysates on (a and b) birchwood glucuronoxylan and (c and d) insoluble oat spelt arabinoxylan by (a and c) Nf Xyn11A and (b and d) Ta Xyn10A enzymes. X2, xylobiose; X3, xylotriose; XUXX, MeGlcA3Xyl4; UXX, MeGlcA3Xyl3; XAXX, arabinoxylotetraose; XAX, arabinoxylotriose, AX, arabinoxylobiose. The peaks at about 4 minutes correspond to xylose. The peak of XAXX was tentatively identified from the order to pKa values of different arabinoxylobiose xylooligosaccharide (AXOS) and the shortest AXOS from GH11 xylanases.