Glucose (Glc) yield as a function of enzyme loading for different pretreatment conditions, H
loadings, and enzyme hydrolysis times. The enzymes used for the hydrolysis were mixtures of four commercial enzymes as established for each specific pretreatment condition (that is, H2O2 loading) at an enzyme loading of 15 mg/g glucan and 48 h hydrolysis (see Table 1). (a) Alkaline hydrogen peroxide (AHP) pretreatment and 24 h enzyme hydrolysis. (b) AHP pretreatment and 48 h digestion. (c) Ammonia fiber expansion (AFEX) pretreatment, 24 h and 48 h enzyme hydrolysis. Biomass loading during the AHP pretreatment was 10%. For AFEX conditions see Methods. All biomass loadings in the enzyme hydrolysis step were 0.2% glucan. Glc yield values indicate yield as a percentage of total measured monomeric Glc content of the biomass. Numbers next to the data points are the Glc yields (y axis values).