Alkaline peroxide pretreatment of corn stover: effects of biomass, peroxide, and enzyme loading and composition on yields of glucose and xylose

Table 1 Optimization of mixtures of four commercial enzymes for monomeric glucose (Glc) and xylose (Xyl) release from corn stover pretreated by alkaline hydrogen peroxide (AHP) or ammonia fiber expansion (AFEX)

Pretreatment	Optimization	Optimal enzyme proportions (%)				Glc or Xyl yield (%)
		Acc 1000	Multifect xylanase	Multifect pectinase	Novozyme 188	MP	Exptl
0.125 g H₂O₂/g biomass	Glc	59	25	16	0	54.0	55.0 ± 1.3
0.25 g H₂O₂/g biomass	Glc	63	14	21	2	86.0	83.0 ± 1.0
0.5 g H₂O₂/g biomass	Glc	64	9	27	0	99.6	95.0 ± 2.4
	Xyl	36	19	45	0	74.0	75.1 ± 0.9
AFEX	Glc	66	34	0	0	62.0	61.5 ± 1.5
	Xyl	40	10	50	0	55.7	53.4 ± 2.1

For all AHP experiments, pretreatment biomass loading was 10% and pretreatment time was 24 h with no pH adjustment during the pretreatment. For AFEX pretreatment conditions see Methods. Enzyme loading was 15 mg/g glucan and digestion time was 48 h. Glc and Xyl values indicate yield as a percentage of total measured monomeric Glc and Xyl content of the biomass. Raw data and statistical analysis are shown in Additional file 1, Supplementary Tables S4-S8.
Acc 1000 = Accellerase 1000; Exptl = experimental results (mean ± 1 SD, n = 8); MP = model prediction.

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