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Table 1 Optimization of mixtures of four commercial enzymes for monomeric glucose (Glc) and xylose (Xyl) release from corn stover pretreated by alkaline hydrogen peroxide (AHP) or ammonia fiber expansion (AFEX)

From: Alkaline peroxide pretreatment of corn stover: effects of biomass, peroxide, and enzyme loading and composition on yields of glucose and xylose

Pretreatment Optimization Optimal enzyme proportions (%) Glc or Xyl yield (%)
   Acc 1000 Multifect xylanase Multifect pectinase Novozyme 188 MP Exptl
0.125 g H2O2/g biomass Glc 59 25 16 0 54.0 55.0 ± 1.3
0.25 g H2O2/g biomass Glc 63 14 21 2 86.0 83.0 ± 1.0
0.5 g H2O2/g biomass Glc 64 9 27 0 99.6 95.0 ± 2.4
  Xyl 36 19 45 0 74.0 75.1 ± 0.9
AFEX Glc 66 34 0 0 62.0 61.5 ± 1.5
  Xyl 40 10 50 0 55.7 53.4 ± 2.1
  1. For all AHP experiments, pretreatment biomass loading was 10% and pretreatment time was 24 h with no pH adjustment during the pretreatment. For AFEX pretreatment conditions see Methods. Enzyme loading was 15 mg/g glucan and digestion time was 48 h. Glc and Xyl values indicate yield as a percentage of total measured monomeric Glc and Xyl content of the biomass. Raw data and statistical analysis are shown in Additional file 1, Supplementary Tables S4-S8.
  2. Acc 1000 = Accellerase 1000; Exptl = experimental results (mean ± 1 SD, n = 8); MP = model prediction.