Alkaline peroxide pretreatment of corn stover: effects of biomass, peroxide, and enzyme loading and composition on yields of glucose and xylose

Table 2 Optimization of mixtures of four commercial enzymes for different alkaline hydrogen peroxide (AHP) pretreatments

Pretreatment conditions	Optimal enzyme proportions (%)				Glc yield (%)
	Acc 1000	Multifect xylanase	Multifect pectinase	Novozyme 188	MP	Exptl
0.125 g H₂O₂/g biomass (10% biomass loading, 24 h, controlled pH)	63	24	13	0	72	73.8 ± 2.2
0.125 g H₂O₂/g biomass (10% biomass loading, 48 h, no pH control)	63	14	20	3	66	66.3 ± 1.3
0.5 g H₂O₂/g biomass (15% biomass loading, 24 h, no pH control)	63	10	27	0	100	96.1 ± 2.1
0.5 g H₂O₂/g biomass (20% biomass loading, 24 h, no pH control)	63	10	27	0	100	96.8 ± 1.0
0.125 g H₂O₂/g biomass (15% biomass loading, 48 h, controlled pH)	62	24	15	0	76	76.8 ± 2.2

All models were optimized for glucose (Glc). Glc values indicate yield as a percentage of total measured Glc content of the biomass. Enzyme loading was 15 mg/g glucan. Raw data and statistical analysis are shown in Additional file 1, Tables S11-S16.
Acc 1000 = Accellerase 1000; Exptl = experimental results (mean ± 1 SD, n = 8); MP = model prediction.

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