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Table 2 Optimization of mixtures of four commercial enzymes for different alkaline hydrogen peroxide (AHP) pretreatments

From: Alkaline peroxide pretreatment of corn stover: effects of biomass, peroxide, and enzyme loading and composition on yields of glucose and xylose

Pretreatment conditions Optimal enzyme proportions (%) Glc yield (%)
  Acc 1000 Multifect xylanase Multifect pectinase Novozyme 188 MP Exptl
0.125 g H2O2/g biomass (10% biomass loading, 24 h, controlled pH) 63 24 13 0 72 73.8 ± 2.2
0.125 g H2O2/g biomass (10% biomass loading, 48 h, no pH control) 63 14 20 3 66 66.3 ± 1.3
0.5 g H2O2/g biomass (15% biomass loading, 24 h, no pH control) 63 10 27 0 100 96.1 ± 2.1
0.5 g H2O2/g biomass (20% biomass loading, 24 h, no pH control) 63 10 27 0 100 96.8 ± 1.0
0.125 g H2O2/g biomass (15% biomass loading, 48 h, controlled pH) 62 24 15 0 76 76.8 ± 2.2
  1. All models were optimized for glucose (Glc). Glc values indicate yield as a percentage of total measured Glc content of the biomass. Enzyme loading was 15 mg/g glucan. Raw data and statistical analysis are shown in Additional file 1, Tables S11-S16.
  2. Acc 1000 = Accellerase 1000; Exptl = experimental results (mean ± 1 SD, n = 8); MP = model prediction.