Figure 5

Conversion of glucan in Avicel to glucose with change in time for adding hemicellulase before (or after) cellulase addition for enzymatic hydrolysis at 50°C and pH 4.8 at an enzyme loading of 5 filter paper units (FPU))/g glucan and 10 cellobiase units (CBU)/g glucan with 2% Avicel glucan. Multifect xylanase and β-xylosidase were supplemented at a loading of 30 mg protein/g equivalent xylose (xylanase: β-xylosidase 2:1). control refers to Avicel (without xylo-oligomers) with an enzyme loading of 5 FPU and 10 CBU/g glucan but no added hemicellulases. The xylo-oligomer control (XOs) was a 2% Avicel loading sample supplemented with 12.5 mg/ml (equivalent xylose concentration) mixed degree of polymerization xylo-oligomers hydrolyzed by cellulase and β-glucosidase at the same dosage as for the control. The xylose sample refers to Avicel hydrolysis with 12.5 mg/ml pure xylose at the same enzyme loadings for comparison.