Supplementation with xylanase and β-xylosidase to reduce xylo-oligomer and xylan inhibition of enzymatic hydrolysis of cellulose and pretreated corn stover

Table 2 Maximum protein-adsorption capacity, affinity constants and correlation coefficients of different enzymes with Avicel, birchwood xylan and solids from corn stover (CS) pretreated with dilute acid and ammonia fiber expansion (AFEX).

Enzyme	Substrates	σ, mg/g substrate¹	Kd, Liter/g²	R ²
Spezyme CP cellulase	Avicel	82.4	1.5	0.98
	Birchwood xylan	99.7	2.5	0.97
	AFEX CS	102.7	2.7	0.94
	Dilute acid CS	92.7	2.8	0.98
Novozyme 188 β-glucosidase	Avicel	52.8	1.3	0.87
	Birchwood xylan	46.3	1.1	0.82
	AFEX CS	78.7	1.8	0.91
	Dilute acid CS	73.6	1.7	0.95
Multifect xylanase	Avicel	60.8	1.9	0.94
	Birchwood xylan	114.1	2.7	0.96
	AFEX CS	85.7	1.8	0.87
	Dilute acid CS	74.3	1.9	0.92
Genencor	Avicel	82.3	2.6	0.95
β-xylosidase	Birchwood xylan	115.2	3.7	0.91
	AFEX CS	98.1	2.3	0.97
	Dilute acid CS	88.5	2.4	0.89

ISSN: 2731-3654