Biotechnology for Biofuels and Bioproducts

Table 1 Tested pretreatment conditions^a

From: Co-hydrolysis of hydrothermal and dilute acid pretreated populus slurries to support development of a high-throughput pretreatment system

Pretreatment			Solids load, % w/w	Severity
Method	Temp, °C	Time, min
Water only	180	17.6	0.5	Log R₀= 3.6^b
Water only	180	44.1	0.5	Log R₀= 4.0
Water only	180	17.6	1.0	Log R₀= 3.6
Water only	180	44.1	1.0	Log R₀= 4.0
Water only	180	17.6	2.0	Log R₀= 3.6
Water only	180	44.1	2.0	Log R₀= 4.0
Sulfuric acid 1% w/w	140	10.3	0.5	Log CS = 1.5^c
Sulfuric acid 1% w/w	140	20.5	0.5	Log CS = 1.8
Sulfuric acid 1% w/w	140	10.3	2.0	Log CS = 1.5
Sulfuric acid 1% w/w	140	20.5	2.0	Log CS = 1.8
Sulfuric acid 2% w/w	140	5.1	2.0	Log CS = 1.5
Sulfuric acid 2% w/w	140	10.3	2.0	Log CS = 1.8

^aPretreatment conditions used to compare co-hydrolysis to the conventional characterization method.
^b, where t denotes time in minutes and T temperature in °C.
^cCS = 10^pH × R_0.

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