SDS-PAGE analysis of the thermostable preparations. Lane 1 = Ta CBHI/Cel7A (6 μg); lane 2 = Ct CBHI/Cel7A (8 μg); lane 3 = At CBHI/Cel7A (5 μg); lane 4 = At EGV/Cel45A (4 μg); lane 5 = protein standards (Bio-Rad 161-0318); lane 6 = Ta EGII/Cel5A (5 μg); lane 7 = Nf XYL/Xyn11A (5 μg); lane 8 = Ta XYL/Xyn10A (5 μg); lane 9 = At βG/Cel3A (4 μg). The Bio-Rad ready-made gel was run at 200V and 100 mA for 1.5 hours and stained with Coomassie for 1 hour. The main protein bands in each preparation representing the heterologously expressed thermostable enzymes are marked. In lane 7, the double band represents the two forms of Nf XYL/Xyn11A: with and without the linker.