Thermostable endoglucanases in the liquefaction of hydrothermally pretreated wheat straw

Table 2 The activities of the enzyme preparations.

Enzyme	Specific activities against different substrates (FPU/mg, nkat/mg)
	FPA	HEC	MUL	BX	PNPG
At CBHI/Cel7A	0.17	10.4	12.6	671	4.2
Ct CBHI/Cel7A	BDL¹	2.0	22.9	72.2	0.1
Ta CBHI/Cel7A	0.05	7.3	10.3	722	2.3
Ta EGII/Cel5A	0.19	1479	BDL¹	6385	20.7
At EGV/Cel45A	BDL¹	95.1	BDL¹	455	1.2
Ta XYL/Xyn10A	BDL¹	6.0	BDL¹	7433	2.6
Nf XYL/Xyn11A	BDL¹	2.5	BDL¹	5651	1.3
At βG/Cel3A	BDL¹	27.3	BDL¹	1602	2348
Celluclast	0.37	129.1	ND²	ND²	ND²
Novozym	ND²	5.2	ND²	ND²	80.5

¹Below detection limit
²Not determined
The hydrolytic activities were measured against filter paper activity (FPA; measuring overall cellulase activity), hydroxyethyl cellulose (HEC; endoglucanase activity), MUL (cellobiohydrolase activity), Birchwood xylan (BX; xylanase activity) and p-nitrophenyl-β- d-glucopyranoside (PNPG; β-glucosidase activity).

ISSN: 2731-3654