Time profiles of fermentations of ILV2 ILV3 ILV5 BAT2 overexpression (a) and reference strain (b). The ILV2 ILV3 ILV5 BAT2 overexpression strain and the reference strain CEN.PK 113-5D were cultivated under anaerobic batch fermentations in mineral medium with 40 g glucose/l. The concentrations of glucose, biomass, and products are plotted as a function of time. Isobutanol concentrations were measured after glucose depletion in both cases. Fermentations were performed in triplicate, representative cultivations are shown.