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Table 1 Effects of gene overexpression on growth rates and product yields under anaerobic batch fermentationsa

From: Increased isobutanol production in Saccharomyces cerevisiae by overexpression of genes in valine metabolism

Strain Reference strainb ILV2 ILV3 ILV5 overexpression strainb ILV2 ILV3 ILV5 BAT2 overexpression strainb ILV2 ILV3 ILV5 ILV6 overexpression strainb
Specific growth rate, per h 0.38 ± 0.02 0.29 ± 0.02 0.16 ± 0.01 0.18 ± 0.00
Biomass (CH1.78O0.6N0.19) yield, g/g glucose 0.10 ± 0.00 0.11 ± 0.01 0.07 ± 0.01 0.11 ± 0.00
Ethanol yield, g/g glucose 0.38 ± 0.01 0.37 ± 0.02 0.34 ± 0.01 0.37 ± 0.00
Pyruvate yield, g/g glucose 0.0011 ± 0.007 0.0016 ± 0.0007 0.0032 ± 0.0006 0.0028 ± 0.0001
Succinate yield, g/g glucose 0.0016 ± 0.0006 0.0006 ± 0.0001 0.0028 ± 0.0001 0.0035 ± 0.0003
Glycerol yield, g/g glucose 0.100 ± 0.008 0.087 ± 0.006 0.095 ± 0.001 0.099 ± 0.003
Acetate yield, g/g glucose 0.0076 ± 0.0014 0.0088 ± 0.0010 0.0110 ± 0.0006 0.0069 ± 0.0003
CO2 yield, g/g glucose 0.32 ± 0.01 0.39 ± 0.01 0.37 ± 0.04 0.41 ± 0.03
Carbon balance deviation,%b 5.9 ± 2.2 3.0 ± 4.0 10.9 ± 1.7 1.3 ± 1.7
  1. aThe mineral medium with 40 g/l of glucose was used.
  2. bThe reference strain used was CEN.PK 113-5D; The ILV2 ILV3 ILV5 overexpression strain used was ILV235_XCY561; The ILV2 ILV3 ILV5 BAT2 overexpression strain used were ILV235BAT2_XCY715 and ILV235BAT2_XCY723; The ILV2 ILV3 ILV5 ILV6 overexpression strain used was ILV2356_XCY605.