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Table 2 Aerobic batch cultivations of the ILV2 ILV3 ILV 5 overexpression strain and reference strain in shake flasksa

From: Increased isobutanol production in Saccharomyces cerevisiae by overexpression of genes in valine metabolism

  Buffered mineral medium YPD complex medium
  Reference stainb ILV2 ILV3 ILV5 overexpression strain b Reference strain ILV2 ILV3 ILV5 overexpression strain
Specific growth rate, per h 0.36 ± 0.00 0.11 ± 0.00 0.52 ± 0.00 0.50 ± 0.00
Biomass (CH1.78O0.6N0.19) yield, g/g glucose 0.33 ± 0.06 0.14 ± 0.05 0.34 ± 0.00 0.35 ± 0.00
Ethanol yield, g/g glucose 0.22 ± 0.05 0.28 ± 0.10 0.12 ± 0.01 0.19 ± 0.02
Pyruvate yield, g/g glucose 0.0002 ± 0.0000 0.0002 ± 0.0000 0.0137 ± 0.0003 0.0134 ± 0.0005
Succinate yield, g/g glucose 0.20 ± 0.02 0.26 ± 0.01 0.02 ± 0.00 0.01 ± 0.00
Glycerol yield, g/g glucose 0.006 ± 0.006 0.026 ± 0.017 0.002 ± 0.000 0.013 ± 0.000
Acetate yield, g/g glucose 0.0000 ± 0.0000 0.0052 ± 0.0022 0.0929 ± 0.0047 0.0929 ± 0.0018
  1. aThe cultivations were carried out in shake flask. Buffered mineral medium containing 40 g/l glucose and YPD complex medium containing 17 g/l glucose were used.
  2. bThe reference strain used was CEN.PK 113-5D. The overexpression strain used was ILV235_XCY561.
  3. YPD = yeast extract/peptone/dextrose.