Plasmid or strain | Relevant characteristics | Source or reference |
---|---|---|
Plasmid | Â | Â |
pCR-Blunt II-TOPO | Cloning vector; Kmr | Invitrogen |
YDp-L | pUC9 derivative, with LEU2 marker | [13] |
YDp-W | pUC9 derivative, with TRP1 marker | [13] |
YDp-H | pUC9 derivative, with HIS3 marker | [13] |
YDp-U | pUC9 derivative, with URA3 marker | [13] |
pTOPO_P+ILV2 | pCR-Blunt II-TOPO with PGK1 promoter and ILV2 gene from Saccharomyces cerevisiae | This study |
pTOPO_P+ILV3 | pCR-Blunt II-TOPO with PGK1 promoter and ILV3 gene from S. cerevisiae | This study |
pTOPO_P+ILV5 | pCR-Blunt II-TOPO with PGK1 promoter and ILV5 gene from S. cerevisiae | This study |
pTOPO_P+ILV6 | pCR-Blunt II-TOPO with PGK1 promoter and ILV6 gene from S. cerevisiae | This study |
pTOPO_P+BAT2 | pCR-Blunt II-TOPO with PGK1 promoter and BAT2 gene from S. cerevisiae | This study |
YDp-L_P+ILV2 | Plasmid YDp-L with PGK1 promoter and ILV2 | This study |
YDp-W_P+ILV3 | Plasmid YDp-W with PGK1 promoter and ILV3 | This study |
YDp-H_P+ILV5 | Plasmid YDp-H with PGK1 promoter and ILV5 | This study |
YDp-U_P+ILV6 | Plasmid YDp-U with PGK1 promoter and ILV6 | This study |
YDp-U_P+BAT2 | Plasmid YDp-U with PGK1 promoter and BAT2 | This study |
Strains | Â | Â |
CEN.PK 2-1C | MAT α leu2-3, 112 his3-Δ1 ura3-52 trp1-289 MAL2-8(Con) MAL3 SUC3 | P Kötter |
CEN.PK 113-5D | MAT α SUC2 MAL2-8c ura3-52 | P Kötter |
CEN.PK 113-7D | MAT α MAL2-8c SUC2 | P Kötter |
ILV235_XCY561 | CEN.PK 2-1C with YDp-H_P+ILV5, YDp-L_P+ILV2, and YDp-W_P+ILV3 inserted into the genome | This study |
ILV235BAT2_XCY715, ILV235BAT2_XCY723 | CEN.PK 2-1C with YDp-H_P+ILV5, YDp-L_P+ILV2, YDp-W_P+ILV3, and YDp-U_P+BAT2 inserted into the genome. | This study |
ILV2356_XCY605 | CEN.PK 2-1C with YDp-H_P+ILV5, YDp-L_P+ILV2, YDp-W_P+ILV3, and YDp-U_P+ILV6 inserted into the genome | This study |