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Table 3 Plasmids and strains

From: Increased isobutanol production in Saccharomyces cerevisiae by overexpression of genes in valine metabolism

Plasmid or strain Relevant characteristics Source or reference
Plasmid   
pCR-Blunt II-TOPO Cloning vector; Kmr Invitrogen
YDp-L pUC9 derivative, with LEU2 marker [13]
YDp-W pUC9 derivative, with TRP1 marker [13]
YDp-H pUC9 derivative, with HIS3 marker [13]
YDp-U pUC9 derivative, with URA3 marker [13]
pTOPO_P+ILV2 pCR-Blunt II-TOPO with PGK1 promoter and ILV2 gene from Saccharomyces cerevisiae This study
pTOPO_P+ILV3 pCR-Blunt II-TOPO with PGK1 promoter and ILV3 gene from S. cerevisiae This study
pTOPO_P+ILV5 pCR-Blunt II-TOPO with PGK1 promoter and ILV5 gene from S. cerevisiae This study
pTOPO_P+ILV6 pCR-Blunt II-TOPO with PGK1 promoter and ILV6 gene from S. cerevisiae This study
pTOPO_P+BAT2 pCR-Blunt II-TOPO with PGK1 promoter and BAT2 gene from S. cerevisiae This study
YDp-L_P+ILV2 Plasmid YDp-L with PGK1 promoter and ILV2 This study
YDp-W_P+ILV3 Plasmid YDp-W with PGK1 promoter and ILV3 This study
YDp-H_P+ILV5 Plasmid YDp-H with PGK1 promoter and ILV5 This study
YDp-U_P+ILV6 Plasmid YDp-U with PGK1 promoter and ILV6 This study
YDp-U_P+BAT2 Plasmid YDp-U with PGK1 promoter and BAT2 This study
Strains   
CEN.PK 2-1C MAT α leu2-3, 112 his3-Δ1 ura3-52 trp1-289 MAL2-8(Con) MAL3 SUC3 P Kötter
CEN.PK 113-5D MAT α SUC2 MAL2-8c ura3-52 P Kötter
CEN.PK 113-7D MAT α MAL2-8c SUC2 P Kötter
ILV235_XCY561 CEN.PK 2-1C with YDp-H_P+ILV5, YDp-L_P+ILV2, and YDp-W_P+ILV3 inserted into the genome This study
ILV235BAT2_XCY715, ILV235BAT2_XCY723 CEN.PK 2-1C with YDp-H_P+ILV5, YDp-L_P+ILV2, YDp-W_P+ILV3, and YDp-U_P+BAT2 inserted into the genome. This study
ILV2356_XCY605 CEN.PK 2-1C with YDp-H_P+ILV5, YDp-L_P+ILV2, YDp-W_P+ILV3, and YDp-U_P+ILV6 inserted into the genome This study