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Table 3 Plasmids and strains

From: Increased isobutanol production in Saccharomyces cerevisiae by overexpression of genes in valine metabolism

Plasmid or strain

Relevant characteristics

Source or reference

Plasmid

  

pCR-Blunt II-TOPO

Cloning vector; Kmr

Invitrogen

YDp-L

pUC9 derivative, with LEU2 marker

[13]

YDp-W

pUC9 derivative, with TRP1 marker

[13]

YDp-H

pUC9 derivative, with HIS3 marker

[13]

YDp-U

pUC9 derivative, with URA3 marker

[13]

pTOPO_P+ILV2

pCR-Blunt II-TOPO with PGK1 promoter and ILV2 gene from Saccharomyces cerevisiae

This study

pTOPO_P+ILV3

pCR-Blunt II-TOPO with PGK1 promoter and ILV3 gene from S. cerevisiae

This study

pTOPO_P+ILV5

pCR-Blunt II-TOPO with PGK1 promoter and ILV5 gene from S. cerevisiae

This study

pTOPO_P+ILV6

pCR-Blunt II-TOPO with PGK1 promoter and ILV6 gene from S. cerevisiae

This study

pTOPO_P+BAT2

pCR-Blunt II-TOPO with PGK1 promoter and BAT2 gene from S. cerevisiae

This study

YDp-L_P+ILV2

Plasmid YDp-L with PGK1 promoter and ILV2

This study

YDp-W_P+ILV3

Plasmid YDp-W with PGK1 promoter and ILV3

This study

YDp-H_P+ILV5

Plasmid YDp-H with PGK1 promoter and ILV5

This study

YDp-U_P+ILV6

Plasmid YDp-U with PGK1 promoter and ILV6

This study

YDp-U_P+BAT2

Plasmid YDp-U with PGK1 promoter and BAT2

This study

Strains

  

CEN.PK 2-1C

MAT α leu2-3, 112 his3-Δ1 ura3-52 trp1-289 MAL2-8(Con) MAL3 SUC3

P Kötter

CEN.PK 113-5D

MAT α SUC2 MAL2-8c ura3-52

P Kötter

CEN.PK 113-7D

MAT α MAL2-8c SUC2

P Kötter

ILV235_XCY561

CEN.PK 2-1C with YDp-H_P+ILV5, YDp-L_P+ILV2, and YDp-W_P+ILV3 inserted into the genome

This study

ILV235BAT2_XCY715, ILV235BAT2_XCY723

CEN.PK 2-1C with YDp-H_P+ILV5, YDp-L_P+ILV2, YDp-W_P+ILV3, and YDp-U_P+BAT2 inserted into the genome.

This study

ILV2356_XCY605

CEN.PK 2-1C with YDp-H_P+ILV5, YDp-L_P+ILV2, YDp-W_P+ILV3, and YDp-U_P+ILV6 inserted into the genome

This study