Conversion of deoxynivalenol to 3-acetyldeoxynivalenol in barley-derived fuel ethanol co-products with yeast expressing trichothecene 3-O-acetyltransferases

Table 4 Final average ethanol (% v/v) for mashes with Ethanol Red, untransformed RW2802 and transformed RW2802 yeasts

Barley line/cultivar	Yeast strain	No galactose	10% Galactose		Ethanol yields
		Final ethanol, % v/v	Final ethanol, % v/v	Final galactose, % v/v	Without galactose. %	With galactose. %
VA06H-25¹	Ethanol Red	9.84	14.43³	0.14	88.49	90.93
	Untransformed RW2802	7.50	7.40	8.37	67.45	46.63
	RW2802 transformed with FgTRI101	7.51	7.57	8.03	67.54	47.70
VA04B-125²	Ethanol Red	7.75	12.79³	0.44	91.41	94.74
	Untransformed RW2802	6.72	6.24	8.78	79.26	46.22
	RW2802 transformed with FgTRI101	7.63	7.68	8.84	90.00	56.89
	RW2802 transformed with FfTRI201	7.74	7.59	8.73	91.30	56.22
Thoroughbred²	Ethanol Red	9.43	14.26³	0.33	83.53	88.96
	Untransformed RW2802	8.48	5.78	8.90	75.11	36.06
	RW2802 transformed with FgTRI101	8.25	6.93	8.77	73.07	43.23
Price²	Ethanol Red	8.27	13.24³	0.39	87.42	92.01
	Untransformed RW2802	6.47	7.37	8.57	68.39	51.22
	RW2802 transformed with FgTRI101	7.93	7.07	8.83	83.83	49.13
Eve¹	Ethanol Red	9.87	13.58³	1.72	85.60	83.62
	Untransformed RW2802	9.49	6.68	9.11	82.31	41.13
	RW2802 transformed with FgTRI101	9.24	6.19	8.94	80.14	38.12

¹Hulless genotype.
²Hulled genotype.
³Using Dry Ethanol Red yeast in mashes with galactose resulted in significantly higher ethanol yields than from the same mash without galactose (P < 0.05). Ethanol yields are also shown for other mashes with and without galactose.

ISSN: 2731-3654