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Table 4 Final average ethanol (% v/v) for mashes with Ethanol Red, untransformed RW2802 and transformed RW2802 yeasts

From: Conversion of deoxynivalenol to 3-acetyldeoxynivalenol in barley-derived fuel ethanol co-products with yeast expressing trichothecene 3-O-acetyltransferases

Barley line/cultivar Yeast strain No galactose 10% Galactose Ethanol yields
   Final ethanol, % v/v Final ethanol, % v/v Final galactose, % v/v Without galactose. % With galactose. %
VA06H-251 Ethanol Red 9.84 14.433 0.14 88.49 90.93
  Untransformed RW2802 7.50 7.40 8.37 67.45 46.63
  RW2802 transformed with FgTRI101 7.51 7.57 8.03 67.54 47.70
VA04B-1252 Ethanol Red 7.75 12.793 0.44 91.41 94.74
  Untransformed RW2802 6.72 6.24 8.78 79.26 46.22
  RW2802 transformed with FgTRI101 7.63 7.68 8.84 90.00 56.89
  RW2802 transformed with FfTRI201 7.74 7.59 8.73 91.30 56.22
Thoroughbred2 Ethanol Red 9.43 14.263 0.33 83.53 88.96
  Untransformed RW2802 8.48 5.78 8.90 75.11 36.06
  RW2802 transformed with FgTRI101 8.25 6.93 8.77 73.07 43.23
Price2 Ethanol Red 8.27 13.243 0.39 87.42 92.01
  Untransformed RW2802 6.47 7.37 8.57 68.39 51.22
  RW2802 transformed with FgTRI101 7.93 7.07 8.83 83.83 49.13
Eve1 Ethanol Red 9.87 13.583 1.72 85.60 83.62
  Untransformed RW2802 9.49 6.68 9.11 82.31 41.13
  RW2802 transformed with FgTRI101 9.24 6.19 8.94 80.14 38.12
  1. 1Hulless genotype.
  2. 2Hulled genotype.
  3. 3Using Dry Ethanol Red yeast in mashes with galactose resulted in significantly higher ethanol yields than from the same mash without galactose (P < 0.05). Ethanol yields are also shown for other mashes with and without galactose.
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Biotechnology for Biofuels

ISSN: 1754-6834

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