Cellulose accessibility limits the effectiveness of minimum cellulase loading on the efficient hydrolysis of pretreated lignocellulosic substrates

Table 2 Coded and actual levels of variables chosen for the statistical design of experiment.

Factors	Level	Pretreatment
		SO₂steam^a		Ethanol organosolv
		CS,^bDF,^cLP^d	CF^e	CF	LP, P^f
Solids loading, %	-1	2	2	2	2
	0	6	6	6	6
	1	10	10	10	10
Hydrolysis time, hours	-1	24	24	24	24
	0	48	48	48	48
	1	72	72	72	72
Cellulase,^g mg protein/g glucan	-1	25	5	13	20
	0	50	15	26	45
	1	75	25	39	70
β-glucosidase,^h mg protein/g glucan	-1	0	0	0	0
	0	15	15	10	10
	1	30	30	20	20

ISSN: 2731-3654