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Table 3 Matrix and results of a 24 full factorial design with centered face and three repetitions at the center point for steam- and organosolv-pretreated lignocellulosic substrates.

From: Cellulose accessibility limits the effectiveness of minimum cellulase loading on the efficient hydrolysis of pretreated lignocellulosic substrates

Run Factors Glucan to glucose, %
Number Time Solids Cell.a BGb SPCFc SPCSd SPLPe SPDFf OPPg OPCFh OPLPi
1 -1 -1 -1 -1 16 46 8 18 12 27 5
2 1 -1 -1 -1 20 59 22 28 12 48 22
3 -1 -1 -1 1 74 62 40 43 27 82 39
4 1 -1 -1 1 79 68 44 46 33 95 57
5 -1 -1 1 -1 31 57 37 44 39 48 27
6 1 -1 1 -1 29 71 60 42 58 82 85
7 -1 -1 1 1 53 73 90 64 88 98 78
8 1 -1 1 1 54 61 72 63 82 76 101
9 -1 1 -1 -1 5 32 16 15 13 14 12
10 1 1 -1 -1 16 42 25 24 26 28 24
11 -1 1 -1 1 52 41 33 33 34 43 34
12 1 1 -1 1 54 46 45 44 45 61 52
13 -1 1 1 -1 24 43 34 35 34 33 30
14 1 1 1 -1 37 53 52 52 56 62 57
15 -1 1 1 1 55 52 53 60 61 69 63
16 1 1 1 1 56 67 63 74 82 90 92
17 0 -1 0 0 78 73 72 70 65 98 84
18 0 1 0 0 59 53 47 52 63 70 68
19 0 0 -1 0 51 61 45 35 42 68 10
20 0 0 1 0 63 64 74 75 82 78 85
21 0 0 0 -1 31 55 40 42 37 43 38
22 0 0 0 1 61 65 71 68 63 91 79
23 -1 0 0 0 63 62 60 61 52 74 62
24 1 0 0 0 63 70 57 72 89 97 91
25 0 0 0 0 58 63 61 67 72 73 67
26 0 0 0 0 68 64 63 62 66 66 77
27 0 0 0 0 60 61 69 65 73 67 74
  1. aCellulase.
  2. bBG = β-glucosidase.
  3. cSPCF = steam-pretreated corn fiber.
  4. dSPCS = steam-pretreated corn stover.
  5. eSPLP = steam-pretreated lodgepole pine.
  6. fSPDF = steam-pretreated douglas fir.
  7. gOPP = organosolv-pretreated poplar.
  8. hOPCF = organosolv-pretreated corn fiber.
  9. iOPLP = organosolv-pretreated lodgepole pine.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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