Biotechnology for Biofuels and Bioproducts

Table 3 Matrix and results of a 2⁴ full factorial design with centered face and three repetitions at the center point for steam- and organosolv-pretreated lignocellulosic substrates.

From: Cellulose accessibility limits the effectiveness of minimum cellulase loading on the efficient hydrolysis of pretreated lignocellulosic substrates

Run	Factors				Glucan to glucose, %
Number	Time	Solids	Cell.^a	BG^b	SPCF^c	SPCS^d	SPLP^e	SPDF^f	OPP^g	OPCF^h	OPLPⁱ
1	-1	-1	-1	-1	16	46	8	18	12	27	5
2	1	-1	-1	-1	20	59	22	28	12	48	22
3	-1	-1	-1	1	74	62	40	43	27	82	39
4	1	-1	-1	1	79	68	44	46	33	95	57
5	-1	-1	1	-1	31	57	37	44	39	48	27
6	1	-1	1	-1	29	71	60	42	58	82	85
7	-1	-1	1	1	53	73	90	64	88	98	78
8	1	-1	1	1	54	61	72	63	82	76	101
9	-1	1	-1	-1	5	32	16	15	13	14	12
10	1	1	-1	-1	16	42	25	24	26	28	24
11	-1	1	-1	1	52	41	33	33	34	43	34
12	1	1	-1	1	54	46	45	44	45	61	52
13	-1	1	1	-1	24	43	34	35	34	33	30
14	1	1	1	-1	37	53	52	52	56	62	57
15	-1	1	1	1	55	52	53	60	61	69	63
16	1	1	1	1	56	67	63	74	82	90	92
17	0	-1	0	0	78	73	72	70	65	98	84
18	0	1	0	0	59	53	47	52	63	70	68
19	0	0	-1	0	51	61	45	35	42	68	10
20	0	0	1	0	63	64	74	75	82	78	85
21	0	0	0	-1	31	55	40	42	37	43	38
22	0	0	0	1	61	65	71	68	63	91	79
23	-1	0	0	0	63	62	60	61	52	74	62
24	1	0	0	0	63	70	57	72	89	97	91
25	0	0	0	0	58	63	61	67	72	73	67
26	0	0	0	0	68	64	63	62	66	66	77
27	0	0	0	0	60	61	69	65	73	67	74

^aCellulase.
^bBG = β-glucosidase.
^cSPCF = steam-pretreated corn fiber.
^dSPCS = steam-pretreated corn stover.
^eSPLP = steam-pretreated lodgepole pine.
^fSPDF = steam-pretreated douglas fir.
^gOPP = organosolv-pretreated poplar.
^hOPCF = organosolv-pretreated corn fiber.
ⁱOPLP = organosolv-pretreated lodgepole pine.

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