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Table 1 Cellobiohydrolases expressed in S. cerevisiae in this study

From: High level secretion of cellobiohydrolases by Saccharomyces cerevisiae

Species and gene name

GenBank accession number

Expression plasmid

Recombinant yeast strain abbreviation

cbh1 encoding genes: expressed under transcriptional control of S. cerevisiae ENO1 promoter

Humicola grisea cbh1 a, b

[GenBank:CAA35159]

pRDH103

Sc[H.g.cbh1]

Thermoascus aurantiacus cbh1 a, b

[GenBank:AAL83303]

pRDH104

Sc[T.a.cbh1]

Talaromyces emersonii cbh1 a, b

[GenBank:AAL89553]

pRDH105

Sc[T.e.cbh1]

Neosartorya fischeri cbh1 c, d

[GenBank:XP_001258278]

pRnD317

Sc[N.f.cbh1]

Penicillium janthinellum cbh1 c, d

[GenBank:X59054.1]

pRnD353

Sc[P.j.cbh1]

Gibberella zeae cbh1 c, d

[GenBank:AY196784.2]

pRnD318

Sc[G.z.cbh1]

Nectria haematococca cbh1 c, d

[GenBank:AY502070.1]

pRnD319

Sc[N.h.cbh1]

Fusarium poae cbh1 c, d

[GenBank:AY706934]

pRnD320

Sc[F.p.cbh1]

Aspergillus terreus cbh1 c, d

[GenBank:XM_001214180]

pRnD322

Sc[As.t.cbh1]

Penicillium chrysogenum cbh1 c, d

[GenBank:AY790330]

pRnD323

Sc[P.c.cbh1]

Neurospora crassa cbh1 c, d

[GenBank:X77778]

pRnD324

Sc[N.c.cbh1]

Chaetomium thermophilum cbh1 b, e

[GenBank:CAM98448.1]

pMI569

Sc[C.t.cbh1]

Acremonium thermophilum cbh1 b, e

[GenBank:CAM98445.1]

pMI567

Sc[Ac.t.cbh1]

Trichoderma reesei cbh1 f

[SwissProt::P62694.1]

pRDH101

Sc[T.r.cbh1]

cbh2 encoding genes: expressed under transcriptional control of S. cerevisiae PGK1 promoter

Cochliobolus heterostrophus C4 cel7b, c

[GenBank:AAM76664.1]

pRDH150

Sc[C.h.cbh2]

Gibberella zeae K59 cel6 b, c

[GenBank:AAQ72468.1]

pRDH151

Sc[G.z.cbh2]

Irpex lacteus MC-2 cex b, c

[GenBank:BAG48183.1]

pRDH152

Sc[I.l.cbh2]

Volvariella volvacea cbhII-I b, c

[GenBank:AAT64008.1]

pRDH153

Sc[V.v.cbh2]

Piromyces sp. E2 cel6A b, c

[GenBank:AAL92497.1]

pRDH154

Sc[P.sp.cbh2]

Talaromyces emersonii cbh2 a, b

[GenBank:AF439936]

pRDH106

Sc[T.e.cbh2]

Trichoderma reesei cbh2 a, f

[SwissProt:P07987.1]

pRDH107

Sc[T.r.cbh2]

Chrysosporium lucknowense cbh2b b, d

[EMBL-Bank::HH793136.1]

pMI574

Sc[C.l.cbh2b]; M0969g

Acremonium cellulolyticus cbh2 b, e

[SwissProt:O93837]

pMI571

Sc[A.c.cbh2]

Chaetomium thermophilum cbh2 b, e

[SwissProt:Q5G2D4]

pMI573

Sc[C.t.cbh2]

  1. aSynthesized by GenScript (Piscataway, NJ, USA); bnative secretion signal; csynthesized by Geneart (Regensburg, Germany); dS. cerevisiae mating factor α-1 precursor secretion signal; esynthesized by Codon Devices (Cambridge, UK); fTrichoderma reesei xyn2 secretion signal; gdiploid ura3Δ/ura3Δ FUR1/fur1Δ strain which has a functional xylose pathway, i.e. over expressed pentose pathway genes and Piromyces xylA, and gre3 deleted.