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Table 3 Combinations of cellobiohydrolases expressed in S. cerevisiae in this study

From: High level secretion of cellobiohydrolases by Saccharomyces cerevisiae

cbh1(under ENO1p/t)

cbh2(under PGK1p/t)

Expression plasmid

Recombinant yeast strain abbreviation

T. emersonii cbh1 a

T. reesei cbh2 b

pRDH109c/pMI578d

Sc[Tecbh1 & Trcbh2]

T. emersonii cbh1-T.r.CBM-C a

T. reesei cbh2 b

pMI553d/pRDH125c

Sc[Tecbh1-TrCBM-C & Trcbh2]

C. thermophilum cbh1 a

T. reesei cbh2 b

pMI579d

Sc[Ctcbh1 & Trcbh Clcbh2b]

H. grisea cbh1 a

T. reesei cbh2 b

pRDH118c/pMI577d

Sc[Hgcbh1 & Trcbh2]

T. aurantiacus cbh1 a

T. reesei cbh2 b

pRDH120c

Sc[Tacbh1 & Trcbh2]

T.r.CBM-N-T. emersonii cbh1 b

T. reesei cbh2 b

pRDH123c

Sc[Tecbh1-TrCBM-N & Trcbh2]

T. emersonii cbh1 a

C. lucknowense cbh2b a

pMI581d

Sc[Tecbh1 & Clcbh2b]

C. thermophilum cbh1 a

C. lucknowense cbh2b a

pMI583d

Sc[Ctcbh1 & Clcbh2b]

T. emersonii cbh1-T.r.CBM a

C. lucknowense cbh2b a

pRDH138c/pMI580d

Sc[Tecbh1-TrCBM-C &Clcbh2b]

H. grisea cbh1 a

C. lucknowense cbh2b a

pRDH140c/pMI582d

Sc[Hgcbh1 & Clcbh2b]

  1. a Native signal sequence; b Trichoderma reesei xyn2 signal sequence; c The ENO1p-cbh1-ENO1t and PGK1p-cbh2-PGK1t expression cassettes are oriented head to tail; d The ENO1p-cbh1-ENO1t and PGK1p-cbh2-PGK1t expression cassettes are oriented tail to tail.