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Table 3 Combinations of cellobiohydrolases expressed in S. cerevisiae in this study

From: High level secretion of cellobiohydrolases by Saccharomyces cerevisiae

cbh1(under ENO1p/t) cbh2(under PGK1p/t) Expression plasmid Recombinant yeast strain abbreviation
T. emersonii cbh1 a T. reesei cbh2 b pRDH109c/pMI578d Sc[Tecbh1 & Trcbh2]
T. emersonii cbh1-T.r.CBM-C a T. reesei cbh2 b pMI553d/pRDH125c Sc[Tecbh1-TrCBM-C & Trcbh2]
C. thermophilum cbh1 a T. reesei cbh2 b pMI579d Sc[Ctcbh1 & Trcbh Clcbh2b]
H. grisea cbh1 a T. reesei cbh2 b pRDH118c/pMI577d Sc[Hgcbh1 & Trcbh2]
T. aurantiacus cbh1 a T. reesei cbh2 b pRDH120c Sc[Tacbh1 & Trcbh2]
T.r.CBM-N-T. emersonii cbh1 b T. reesei cbh2 b pRDH123c Sc[Tecbh1-TrCBM-N & Trcbh2]
T. emersonii cbh1 a C. lucknowense cbh2b a pMI581d Sc[Tecbh1 & Clcbh2b]
C. thermophilum cbh1 a C. lucknowense cbh2b a pMI583d Sc[Ctcbh1 & Clcbh2b]
T. emersonii cbh1-T.r.CBM a C. lucknowense cbh2b a pRDH138c/pMI580d Sc[Tecbh1-TrCBM-C &Clcbh2b]
H. grisea cbh1 a C. lucknowense cbh2b a pRDH140c/pMI582d Sc[Hgcbh1 & Clcbh2b]
  1. a Native signal sequence; b Trichoderma reesei xyn2 signal sequence; c The ENO1p-cbh1-ENO1t and PGK1p-cbh2-PGK1t expression cassettes are oriented head to tail; d The ENO1p-cbh1-ENO1t and PGK1p-cbh2-PGK1t expression cassettes are oriented tail to tail.