Adsorption isotherm of purified swollenin onto filter paper. Thepredicted Langmuir isotherm, according to Equation (1), is shown as a solidline (R2 = 0.91) and corresponding parameters (includingstandard deviations) are: A
(swollenin) = 0.089 ±0.006 μmol/g, K
(swollenin) = 0.707 ± 0.196μmol/L. The initial swollenin concentration, added at the start of theincubation, is also shown for a better understanding of Figure 8; 20 g/LWhatman filter paper No.1 in 0.05 M sodium acetate buffer at pH 4.8, T = 45°C, V
= 1 mL, n = 1000 rpm,d
= 3 mm, incubation time 2 h.