Photography and light microscopy of filter paper after pretreatment withswollenin. (A) Macroscopic pictures of pretreated filter paper inpetri dishes and number of agglomerates. All pretreatments were initiated withthe same initial number (80) of filter paper agglomerates (initial diameterapprox. 3 mm). Number of agglomerates (> 0.5 mm) was measured by imageanalysis; (B) Light microscopy of pretreated filter paper. Eclipse E600(Nikon); Pretreatment: 20 g/L cellulose in 0.05 M sodium acetate buffer at pH4.8, 0.4 g/L BSA (approx. 6 μmol/L) or swollenin (approx. 5 μmol/L),T = 45°C, V
= 1 mL, n = 1000rpm, d
= 3 mm, incubation time 48 h.