Figure 5
Particle size of cellulosic substrates after pretreatment withswollenin. (A, B, C, D) Volumetric particle-size distribution ofpretreated cellulosic substrates: (A) Whatman filter paper No.1;(B) α-Cellulose; (C) Avicel PH101; (D) Sigmacell101; (E, F, G, H) Geometric mean particle size of pretreated cellulosicsubstrates: (E) Whatman filter paper No.1; (F) α-Cellulose;(G) Avicel PH101; (H) Sigmacell 101. Errors are given asstandard deviations; Pretreatment: 20 g/L cellulose in 0.05 M sodium acetatebuffer at pH 4.8, 0.4 g/L BSA (approx. 6 μmol/L) or 0.4 g/L swollenin(approx. 5 μmol/L), T = 45°C, V L = 1 mL,n = 1000 rpm, d 0 = 3 mm, incubation time 48 h.Particles (< 2 mm) were analyzed using the particle size analyzer LS13320(Beckman Coulter).