Figure 7
Hydrolysis of cellulosic substrates after pretreatment with swollenin.(A) Whatman filter paper No.1; (B) α-Cellulose;(C) Avicel PH101; (D) Sigmacell 101. Errors are given as standarddeviations; Pretreatment: 20 g/L cellulose in 0.05 M sodium acetate buffer atpH 4.8, 0.4 g/L BSA (approx. 6 μmol/L) or 0.4 g/L swollenin (approx. 5μmol/L), T = 45°C, V L = 1 mL,n = 1000 rpm, d 0 = 3 mm, incubation time 48 h;Hydrolysis: 10 g/L pretreated cellulose in 0.05 M sodium acetate buffer at pH4.8, 1 g/L rebuffered Celluclast®, T = 45°C,V L = 1 mL, n = 1000 rpm, d 0 = 3 mm.