Figure 1

Schematic diagram of simultaneous saccharification and fermentation (SSF) using cellulose of paper sludge (PS) origin and thermotolerant Saccharomyces cerevisiae TJ14. PS was used as a carbon source for cellulase production by Acremonium cellulolyticus C-1 at 28°C. The culture broth containing cellulase was separated from the A. cellulolyticus culture and used for saccharification of PS in SSF at 42°C. Ethanol fermentation was carried out by inoculation with yeast simultaneously with saccharification of PS during SSF. After SSF, the ethanol solution was separated from the SSF culture broth.