The effect of enzyme concentration on the release of saccharification products from cellulose over time as determined by Cellulase 4D. (A) Rate of glucose production over time from cellulose treated with various concentrations of cellulase mixture. The base case covering the concentrations of all enzymes is indicated by the number 1 and proportions of this amount given in the legend (B) Rate of glucose release at varying endoglucanase concentrations (between 800 and 50 mM) where the amounts of other cellulases are added as per the base case. (C) Cellobiose production rates under the same conditions described in (B). The legend showing different enzyme concentration (mM) settings used in the simulation is shown to the right of each graph and base case values are given in Table 1.