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Table 1 Predicted Aspergillus niger cellulase-encoding genes that have increased mRNA accumulation during growth on steam-exploded sugarcane bagasse compared to growth on fructose reference control

From: Transcriptome analysis of Aspergillus niger grown on sugarcane bagasse

Gene locus Motifs* GH family SP MS Description log2 Cy5/Cy3 ratio**
  Sequence Location Orientation      6 hours 12 hours 24 hours
An03g03740     GH1 No No β-glucosidase (bgl4) 4.22 3.53 4.04
An11g02100 GGCTAG 249 1 GH1 Yes No β-glucosidase    
An15g04800 TTAGCC -178 -1 GH3 Yes No β-1.2-D-glucosidase (b2tom) 1.90 0.37 1.85
An07g07630 GGCTAA -180 1 GH3 Yes No β-glucosidase 4.88 3.67 3.21
An17g00520     GH3 No No β-glucosidase precursor (bgluc) 5.34 5.49 5.23
An18g03570     GH3 Yes Yes β-glucosidase (bgl1) 6.06 3.55 4.31
An11g00200     GH3 Yes No β-glucosidase (bgln) 6.23 5.34 5.94
An11g06090 CTAGCC
GGCTAA
-69
-482
-1
1
GH3 Yes No β-glucosidase 2 (bgl2) 5.44 4.62 3.19
An03g01050     GH5 Yes No Endo-β-1.4-glucanase 3.75 0.92 2.58
An01g11670     GH5 Yes No Endo-β-1.4-glucanase A (eglA) 6.25 6.97 6.86
An07g08950 GGCTAA -128 1 GH5 Yes No Endo-β-1.4-glucanase B 8.21 8.03 8.16
An08g01760 TTAGCC -185 -1 GH6 Yes No Exocellobiohydrolase 5.07 3.47 2.73
An12g02220     GH6 Yes Yes Cellulose 1.4-β-cellobiosidase II (cbh2) 6.03 6.15 7.36
An07g09330     GH7 Yes No 1.4-β-D-glucan cellobiohydrolase A precursor (cbhA) 5.95 5.83 3.89
An01g11660 GGCTAG TTAGCC -417
-157
1
1
GH7 Yes No 1.4-β-D-glucan cellobiohydrolase B precursor (cbhB) 7.43 6.91 6.58
An14g02760     GH12 Yes No Endoglucanase A (eglA) 7.88 6.85 6.35
An12g04610     GH61 Yes No Similarity to endoglucanase IV (egl4) 6.78 6.40 6.59
An01g01870 GGCTAA -394 1 GH74 Yes No Avicelase III 4.69 4.52 4.15
  1. GH = glycoside hydrolase; SP = signal peptide; MS = mass spectrometry; log2 Cy5/Cy3 ratio = base 2 logarithm ratio of cyanine 5 to cyanine 3. *Search of the promoter regions (500 bp upstream of putative translational start codon) of the displayed genes for the A. niger XlnR-binding sites containing either 5'-TTAGCC-3' or 5'-GGCTAG-3'. Location is relative to start codon. **log2 Cy5/Cy3 ratio observed in microarray hybridizations.