Transcriptome analysis of Aspergillus niger grown on sugarcane bagasse

Table 1 Predicted Aspergillus niger cellulase-encoding genes that have increased mRNA accumulation during growth on steam-exploded sugarcane bagasse compared to growth on fructose reference control

Gene locus	Motifs*			GH family	SP	MS	Description	log₂ Cy5/Cy3 ratio**
	Sequence	Location	Orientation					6 hours	12 hours	24 hours
An03g03740				GH1	No	No	β-glucosidase (bgl4)	4.22	3.53	4.04
An11g02100	GGCTAG	249	1	GH1	Yes	No	β-glucosidase
An15g04800	TTAGCC	-178	-1	GH3	Yes	No	β-1.2-D-glucosidase (b2tom)	1.90	0.37	1.85
An07g07630	GGCTAA	-180	1	GH3	Yes	No	β-glucosidase	4.88	3.67	3.21
An17g00520				GH3	No	No	β-glucosidase precursor (bgluc)	5.34	5.49	5.23
An18g03570				GH3	Yes	Yes	β-glucosidase (bgl1)	6.06	3.55	4.31
An11g00200				GH3	Yes	No	β-glucosidase (bgln)	6.23	5.34	5.94
An11g06090	CTAGCC GGCTAA	-69 -482	-1 1	GH3	Yes	No	β-glucosidase 2 (bgl2)	5.44	4.62	3.19
An03g01050				GH5	Yes	No	Endo-β-1.4-glucanase	3.75	0.92	2.58
An01g11670				GH5	Yes	No	Endo-β-1.4-glucanase A (eglA)	6.25	6.97	6.86
An07g08950	GGCTAA	-128	1	GH5	Yes	No	Endo-β-1.4-glucanase B	8.21	8.03	8.16
An08g01760	TTAGCC	-185	-1	GH6	Yes	No	Exocellobiohydrolase	5.07	3.47	2.73
An12g02220				GH6	Yes	Yes	Cellulose 1.4-β-cellobiosidase II (cbh2)	6.03	6.15	7.36
An07g09330				GH7	Yes	No	1.4-β-D-glucan cellobiohydrolase A precursor (cbhA)	5.95	5.83	3.89
An01g11660	GGCTAG TTAGCC	-417 -157	1 1	GH7	Yes	No	1.4-β-D-glucan cellobiohydrolase B precursor (cbhB)	7.43	6.91	6.58
An14g02760				GH12	Yes	No	Endoglucanase A (eglA)	7.88	6.85	6.35
An12g04610				GH61	Yes	No	Similarity to endoglucanase IV (egl4)	6.78	6.40	6.59
An01g01870	GGCTAA	-394	1	GH74	Yes	No	Avicelase III	4.69	4.52	4.15

GH = glycoside hydrolase; SP = signal peptide; MS = mass spectrometry; log₂ Cy5/Cy3 ratio = base 2 logarithm ratio of cyanine 5 to cyanine 3. *Search of the promoter regions (500 bp upstream of putative translational start codon) of the displayed genes for the A. niger XlnR-binding sites containing either 5'-TTAGCC-3' or 5'-GGCTAG-3'. Location is relative to start codon. **log₂ Cy5/Cy3 ratio observed in microarray hybridizations.

ISSN: 2731-3654