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Table 3 Gene expression measured by real-time RT-PCR of genes encoding Aspergillus niger cellulases, hemicellulases, xylose reductase and xlnR during growth on steam-exploded sugarcane bagasse

From: Transcriptome analysis of Aspergillus niger grown on sugarcane bagasse

Gene and locus Control (fructose) SEB 6 hours SEB 12 hours SEB 24 hours
cbhA (An07g09330) 0.0086 ± 0.0008 0.3178 ± 0.0104 0.0477 ± 0.0013 0.0284 ± 0.0066
cbhB (An01g11660) 0.0023 ± 0.0000 4.2126 ± 0.1298 1.2376 ± 0.0629 1.6419 ± 0.3598
eglA (An01g11670) 0.0008 ± 0.0000 1.4762 ± 0.2621 0.7261 ± 0.0368 0.3397 ± 0.0740
eglB (An07g08950) 0.0062 ± 0.0004 3.7060 ± 0.0112 0.6213 ± 0.0248 0.1227 ± 0.0263
xynB (An01g00780) 0.0039 ± 0.0001 153.0746 ± 2.8399 9.8474 ± 0.6560 6.4386 ± 1.5611
xlnD (An01g09960) 0.0110 ± 0.0003 15.4472 ± 0.1495 0.7838 ± 0.0036 0.0617 ± 0.0149
xlnR (An15g05810) 0.76 ± 0.00 5.93 ± 0.62 1.71 ± 0.06 2.35 ± 0.24
xyrA (An01g03780) 0.33 ± 0.02 268.58 ± 6.22 45.84 ± 1.98 36.40 ± 1.11
  1. SEB = steam-exploded sugarcane bagasse. The mRNA accumulation of several A. niger genes during growth on SEB. Cultures were grown for 24 hours at 30°C on batch cultivation medium (BCM) + 50 mM fructose, then the mycelial mass was transferred to BCM without yeast extract + steam-exploded sugarcane bagasse and grown for 6, 12 and 24 hours at 30°C. Real-time RT-PCR was used to quantify the mRNA. The measured quantity of a specific gene mRNA in each of the treated samples was normalized using the comparative threshold (Ct) values obtained for the actin mRNA amplifications run in the same plate. The relative quantitation of specific gene and actin gene expression was determined by a standard curve (that is, Ct values plotted against logarithm of DNA copy numbers). The results of four sets of experiments were combined for each determination. Data presented are means ± SD. The values represent the cDNA concentration of a specific gene divided by the actin cDNA concentration.