Transcriptome analysis of Aspergillus niger grown on sugarcane bagasse

Table 3 Gene expression measured by real-time RT-PCR of genes encoding Aspergillus niger cellulases, hemicellulases, xylose reductase and xlnR during growth on steam-exploded sugarcane bagasse

Gene and locus	Control (fructose)	SEB 6 hours	SEB 12 hours	SEB 24 hours
cbhA (An07g09330)	0.0086 ± 0.0008	0.3178 ± 0.0104	0.0477 ± 0.0013	0.0284 ± 0.0066
cbhB (An01g11660)	0.0023 ± 0.0000	4.2126 ± 0.1298	1.2376 ± 0.0629	1.6419 ± 0.3598
eglA (An01g11670)	0.0008 ± 0.0000	1.4762 ± 0.2621	0.7261 ± 0.0368	0.3397 ± 0.0740
eglB (An07g08950)	0.0062 ± 0.0004	3.7060 ± 0.0112	0.6213 ± 0.0248	0.1227 ± 0.0263
xynB (An01g00780)	0.0039 ± 0.0001	153.0746 ± 2.8399	9.8474 ± 0.6560	6.4386 ± 1.5611
xlnD (An01g09960)	0.0110 ± 0.0003	15.4472 ± 0.1495	0.7838 ± 0.0036	0.0617 ± 0.0149
xlnR (An15g05810)	0.76 ± 0.00	5.93 ± 0.62	1.71 ± 0.06	2.35 ± 0.24
xyrA (An01g03780)	0.33 ± 0.02	268.58 ± 6.22	45.84 ± 1.98	36.40 ± 1.11

SEB = steam-exploded sugarcane bagasse. The mRNA accumulation of several A. niger genes during growth on SEB. Cultures were grown for 24 hours at 30°C on batch cultivation medium (BCM) + 50 mM fructose, then the mycelial mass was transferred to BCM without yeast extract + steam-exploded sugarcane bagasse and grown for 6, 12 and 24 hours at 30°C. Real-time RT-PCR was used to quantify the mRNA. The measured quantity of a specific gene mRNA in each of the treated samples was normalized using the comparative threshold (C_t) values obtained for the actin mRNA amplifications run in the same plate. The relative quantitation of specific gene and actin gene expression was determined by a standard curve (that is, C_t values plotted against logarithm of DNA copy numbers). The results of four sets of experiments were combined for each determination. Data presented are means ± SD. The values represent the cDNA concentration of a specific gene divided by the actin cDNA concentration.

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