Transcriptome analysis of Aspergillus niger grown on sugarcane bagasse

Table 4 Predicted Aspergillus niger transporter-encoding genes that have increased mRNA accumulation during growth on steam-exploded sugarcane bagasse compared to growth on fructose reference control

Gene locus	Motifs*			Description	log₂ Cy5/Cy3 ratio**
	Sequence	Location	Orientation		6 hours	12 hours	24 hours
An01g00850	CTAGCC GGCTAA	-376 -385	-1 1	Similarity to xylose permease (xylT)	4.04	3.91	3.79
An06g00260	CTAGCC	-49	-1	Strong similarity to hexose transporter (hxt5)	5.87	4.36	6.23
An06g00620				Strong similarity to α-glucoside-hydrogen symporter (mal11)	7.19	4.73	5.96
An11g03700				Strong similarity to hexose transporter (hxt1)	1.33	1.97	2.64
An12g09270	GGCTAA	-356	1	Strong similarity to lactose permease (lac12)	3.52	4.44	2.94
An15g04270				Strong similarity to quinate transport protein (qutD)	5.07	6.02	8.29
An15g05440	TTAGCC	-6	-1	Strong similarity to high-affinity glucose transporter (hgt1)	3.85	3.02	2.86

log₂ Cy5/Cy3 ratio = base 2 logarithm ratio of cyanine 5 to cyanine 3. *Results of a search of the promoter regions (500 bp upstream of putative translational start codon) of displayed genes for A. niger XlnR-binding sites containing either 5'-TTAGCC-3' or 5'-GGCTAG-3'. Location relative to start codon. **log₂ Cy5/Cy3 ratio observed in microarray hybridizations.

ISSN: 2731-3654