Transcriptome analysis of Aspergillus niger grown on sugarcane bagasse

Table 5 Gene expression measured by real-time RT-PCR of genes encoding Aspergillus niger putative transporters during growth on steam-exploded sugarcane bagasse

Gene locus	Control (fructose)	SEB 6 hours	SEB 12 hours	SEB 24 hours
An01g00850	0.064 ± 0.007	1.41 ± 0.01	1.38 ± 0.29	0.10 ± 0.02
An06g00260	0.21 ± 0.03	0.89 ± 0.09	1.70 ± 0.30	0.22 ± 0.02
An06g00620	2.25 ± 0.25	8.96 ± 0.93	8.87 ± 1.15	1.49 ± 0.19
An11g03700	0.396 ± 0.017	2.49 ± 0.08	3.02 ± 0.32	0.094 ± 0.019
An12g09270	0.96 ± 0.08	23.40 ± 3.50	4.28 ± 0.64	3.17 ± 0.61
An15g04270	0.036 ± 0.004	0.037 ± 0.006	0.13 ± 0.02	0.110 ± 0.009
An15g05440	0.021 ± 0.004	0.071 ± 0.002	0.041 ± 0.007	0.003 ± 0.001

SEB = steam-exploded sugarcane bagasse. The mRNA accumulation of several A. niger transporter-encoding genes during growth on SEB is shown. Cultures were grown for 24 hours at 30°C on batch cultivation medium (BCM) + 50 mM fructose, and the mycelia were transferred to BCM without yeast extract + SEB and grown for 6, 12, and 24 hours at 30°C. Real-time RT-PCR was used to quantify the mRNA. The measured quantity of a specific gene mRNA in each of the treated samples was normalized using the comparative threshold (C_t) values obtained for the actin mRNA amplifications run in the same plate. The relative quantitation of a specific gene and actin gene expression was determined by drawing a standard curve (that is, C_t values plotted against logarithm of DNA copy numbers). The results of four sets of experiments were combined for each determination. Data presented are means ± SD. The values represent the cDNA concentration of a specific gene divided by the actin cDNA concentration.

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