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Table 5 Gene expression measured by real-time RT-PCR of genes encoding Aspergillus niger putative transporters during growth on steam-exploded sugarcane bagasse

From: Transcriptome analysis of Aspergillus niger grown on sugarcane bagasse

Gene locus Control (fructose) SEB 6 hours SEB 12 hours SEB 24 hours
An01g00850 0.064 ± 0.007 1.41 ± 0.01 1.38 ± 0.29 0.10 ± 0.02
An06g00260 0.21 ± 0.03 0.89 ± 0.09 1.70 ± 0.30 0.22 ± 0.02
An06g00620 2.25 ± 0.25 8.96 ± 0.93 8.87 ± 1.15 1.49 ± 0.19
An11g03700 0.396 ± 0.017 2.49 ± 0.08 3.02 ± 0.32 0.094 ± 0.019
An12g09270 0.96 ± 0.08 23.40 ± 3.50 4.28 ± 0.64 3.17 ± 0.61
An15g04270 0.036 ± 0.004 0.037 ± 0.006 0.13 ± 0.02 0.110 ± 0.009
An15g05440 0.021 ± 0.004 0.071 ± 0.002 0.041 ± 0.007 0.003 ± 0.001
  1. SEB = steam-exploded sugarcane bagasse. The mRNA accumulation of several A. niger transporter-encoding genes during growth on SEB is shown. Cultures were grown for 24 hours at 30°C on batch cultivation medium (BCM) + 50 mM fructose, and the mycelia were transferred to BCM without yeast extract + SEB and grown for 6, 12, and 24 hours at 30°C. Real-time RT-PCR was used to quantify the mRNA. The measured quantity of a specific gene mRNA in each of the treated samples was normalized using the comparative threshold (Ct) values obtained for the actin mRNA amplifications run in the same plate. The relative quantitation of a specific gene and actin gene expression was determined by drawing a standard curve (that is, Ct values plotted against logarithm of DNA copy numbers). The results of four sets of experiments were combined for each determination. Data presented are means ± SD. The values represent the cDNA concentration of a specific gene divided by the actin cDNA concentration.