Figure 4From: Application of a Burkholderia cepacialipase-immobilized silica monolith to batch and continuous biodiesel production with a stoichiometric mixture of methanol and crude Jatropha oilEffect of washing of used immobilized lipase during its repeated use. First reaction (open circles), second reaction after washing with acetone (open squares), second reaction after washing with ter-butanol (open diamonds), second reaction without washing (closed circles). Methanol:oil molar ratio, 3:1; water content, 0.6% (w/w).Back to article page