Figure 3

Inhibitory effects of xylan oligosaccharide on the para -nitrophenyl-lac activity of Tr Cel7A. The inhibitory effects of high xylan oligosaccharide (XOS) (open squares) and low XOS (open circles) on the para-nitrophenyl-lacoside (pNP-lac) hydrolytic activity of cellobiohydrolase I (TrCel7A) at 50°C are shown. The molarities based on the estimates measured by high-performance anion-exchange chromatography with pulsed amperometric detection analysis (see Figure 1), 0.5 g/L high XOS and 0.5 g/L low XOS, were estimated to be 0.47 mM and 0.88 mM, respectively. The error bars represent the standard deviations of three experiments.