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Table 2 Binding constants based on a molar concentration scale of TrCel7A and TrCel7A core to linear xylan oligosaccharides

From: Xylan oligosaccharides and cellobiohydrolase I (TrCel7A) interaction and effect on activity

Xylan oligosaccharide

TrCel7A core (K)

TrCel7A (K)

Xylotriose (xyl)3

> 1.0 × 103

-

Xylotetraose (xyl)4

2.97 × 103

-

Xylohexaose (xyl)5

3.44 × 103

3.44 × 103

Xyloheptaose (xyl)7

7.43 × 103

5.76 × 103

Xylooctaose/xylononaose, (xyl)8/(xyl)9 (1:1)

1.19 × 104

9.20 × 104

Xylooctaose/xylononaose/xylodecaose, (xyl)8/(xyl)9/(xyl)10 (1:1:1)

2.91 × 105

-

Low XOS (5 g/L and 8.4 mM, average 4 DP)a

7.18 × 102

9.13 × 102

High XOS (5 g/L and 4.4 mM, average 8 DP)a

4.57 × 103

3.47 × 103

  1. DP = degree of polymerisation; TrCel7A = cellobiohydrolase I; XOS = xylan oligosaccharide; - = no data. aEstimated on the basis of high-performance anion-exchange chromatography with pulsed amperometric detection traces and binding experiments (n = 3).