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Table 2 Binding constants based on a molar concentration scale of TrCel7A and TrCel7A core to linear xylan oligosaccharides

From: Xylan oligosaccharides and cellobiohydrolase I (TrCel7A) interaction and effect on activity

Xylan oligosaccharide TrCel7A core (K) TrCel7A (K)
Xylotriose (xyl)3 > 1.0 × 103 -
Xylotetraose (xyl)4 2.97 × 103 -
Xylohexaose (xyl)5 3.44 × 103 3.44 × 103
Xyloheptaose (xyl)7 7.43 × 103 5.76 × 103
Xylooctaose/xylononaose, (xyl)8/(xyl)9 (1:1) 1.19 × 104 9.20 × 104
Xylooctaose/xylononaose/xylodecaose, (xyl)8/(xyl)9/(xyl)10 (1:1:1) 2.91 × 105 -
Low XOS (5 g/L and 8.4 mM, average 4 DP)a 7.18 × 102 9.13 × 102
High XOS (5 g/L and 4.4 mM, average 8 DP)a 4.57 × 103 3.47 × 103
  1. DP = degree of polymerisation; TrCel7A = cellobiohydrolase I; XOS = xylan oligosaccharide; - = no data. aEstimated on the basis of high-performance anion-exchange chromatography with pulsed amperometric detection traces and binding experiments (n = 3).