Skip to main content

Table 4 Cellobiose release from phosphoric acid swollen cellulose by TrCel7A and TrCel7A core at 50°C and 30°C

From: Xylan oligosaccharides and cellobiohydrolase I (TrCel7A) interaction and effect on activity

Enzyme (temperature substrate)

Positive control

1 g/L low XOS

1 g/L high XOS

TrCel7A core, 50°C

457 ± 10 μM cb

260 ± 19 μM cb

196 ± 3 μM cb

PASC

100%

57%

43%

TrCel7A, 50°C

695 ± 65 μM cb

611 ± 60 μM cb

587 ± 57 μM cb

PASC

100%

88%

85%

TrCel7A core, 30°C

183 ± 7 μM cb

111 ± 8 μM cb

87 ± 5 μM cb

PASC

100%

61%

48%

TrCel7A, 30°C

194 ± 8 μM cb

194 ± 4 μM cb

196 ± 1 μM cb

PASC

100%

96%

100%

  1. cb: cellobiose-solubilised; PASC = phosphoric acid swollen cellulose; TrCel7A = cellobiohydrolase I; XOS = xylan oligosaccharide. The deviations are the standard deviations of five independent experiments.