Figure 2

Enzymatic saccharification of whole rice plants in the solid-state fermentation model system. (A) Free sugar (sum of glucose, fructose, and sucrose) produced from whole rice plants by the addition of various concentrations of ACS cellulase. ACS addition amounts were 1.29 FPU/g DM (black square), 0.86 FPU/g DM (black triangle), 0.43 FPU/g DM (black circle), 0.086 FPU/g DM (open triangle), 0.022 FPU/g DM (open circle), and without enzyme addition (open square). (B) Free sugar (sum of glucose, fructose, and sucrose) produced from whole rice plants by the addition of 0.86 FPU/g DM of various cellulases. ACS (black triangle), cellulase from T. viride (open circle), and cellulase from T. reesei (black circle). (C) Free sugar (sum of glucose, fructose, and sucrose) produced from whole rice plants by the addition of a combination of ACS and glucoamylase. 0.86 FPU/g DM ACS and 3.2 U/g DM glucoamylase (open circle), 0.86 FPU/g DM ACS and 0.32 U/g DM glucoamylase (black square), 0.86 FPU/g DM ACS (black triangle), and without enzyme addition (black circle). Values are expressed as the mean (SD) (n = 3). Values with different lowercase letters are significantly different (P < 0.05) as determined by analysis of variance. ACS: cellulase from Acremonium cellulolyticus; DM: dry matter; FPU: filter paper unit.