Figure 1From: A strain of Saccharomyces cerevisiaeevolved for fermentation of lignocellulosic biomass displays improved growth and fermentative ability in high solids concentrations and in the presence of inhibitory compoundsEffect of solids loading on Saccharomyces cerevisiae strain XR122N. (A) Freeze-dried XR122N was inoculated at an initial concentration of 4 g/L cell dry weight (dcw) into small-scale bioreactors containing pretreated pine at a solids loading of 5% (green diamonds), 10% (blue circles), and 12% (red triangles) w/v. Cellulases and cellobiase were added simultaneously with the inoculum (15 FPU cellulase, and 60 CU cellobiase per gdw of pretreated pine). (B) XR122N was inoculated into 12% w/v solids loading of pretreated pine in a freeze-dried state at an initial concentration of 4 g/L dcw (black squares), 2.0 g/L dcw (red diamonds), 1.0 g/L dcw (blue circles), or 0.5 g/L dcw (green diamonds). Fermentations were maintained at 35°C, pH 5.0, and performed in triplicate. Error bars represent one standard deviation from the mean. FPU, filter paper units; dcw, cell dry weight; gdw, gram dry weight; CU, cellobiase units.Back to article page