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Table 2 Effect of the expression of the Anp1, Bud31, Hpr1, Pho85, Ppa1, Rpl1B, Vrp1 and Ygl024w genes, required for tolerance to inhibitory concentrations of glucose, acetic acid and ethanol, in VHG fermentation.

From: Identification of candidate genes for yeast engineering to improve bioethanol production in very high gravity and lignocellulosic biomass industrial fermentations

Strain [Ethanol] (g/L) ΔEthanol (compared to wild-type cells) [CO2] at mid-fermentation (g/L) ΔCO2 (compared to wild-type cells)
BY4741 136 ± 2 0 72 ± 5 0
Δanp1 122 ± 1 -11 ± 1** 67 ± 1 0 ± 4
Δbud31 70 ± 1 -49 ± 1** 14 ± 0 -54 ± 3**
Δhpr1 75 ± 1 -45 ± 1** 28 ± 0 -41 ± 3**
Δpho85 108 ± 0 -21 ± 1** 57 ± 5 -12 ± 6*
Δrpl1b 132 ± 5 -3 ± 3 66 ± 1 -1 ± 4
Δvrp1 117 ± 2 -13 ± 2** 64 ± 1 -16 ± 2**
Δvgl024w 126 ± 1 -8 ± 1** 55 ± 1 -18 ± 3**
Δppa1 127 ± 1 -7 ± 1** 84 ± 1 25 ± 3**
  1. The comparison of the fermentation profile of wild-type cells and of the deletion mutants was based on the concentration of ethanol produced at the end of the fermentation ([Ethanol] and ΔEthanol) and on the amount of CO2 formed ([CO2] and ΔCO2) at mid-fermentation point (49 h - time taken by the parental strain to reach 50% of the total CO2 produced), as described in Methods. The results shown are means of at least two independent experiments and the statistical significance of the results obtained was quantified using a t-test (n = 2). *P <0.05; **P < 0.01.