SDS-PAGE analysis of leaf soluble protein with or without prior heat pretreatment. Total leaf soluble protein was extracted from newly matured leaves of six independent T0 transgenic (#1 to #6) and wild type (WT) rice plants (A) without or (B) with prior heat treatment at 78°C for 45 min before electrophoretic separation by SDS-PAGE. Twenty micrograms of protein was loaded per lane. M: protein standards. After electrophoresis, gels were stained with Coomassie Brilliant Blue R-250.