Figure 6From: High level expression of Acidothermus cellulolyticusβ-1, 4-endoglucanase in transgenic rice enhances the hydrolysis of its straw by cultured cow gastric fluidSpecific activities of E1 enzyme in different organs. Specific activities of (A) E1 in the leaves of six independent T0 transgenic line (#1 to #6) and wild type (WT) rice plants, (B) in the mature seed (MS), green floret (F), leaf (L), stem (S) and root (R) of T0 transgenic line #3-5, and (C) in the leaves of some T1 plants of transgenic line #3-5. Enzyme activity was assayed using a fluorescence spectrophotometer by its ability to cleave 4-methylumbelliferyl β-D-cellobioside to produce the fluorophore, 4-methylumbelliferne with peak excitation wavelength at 365 nm and peak fluorescence at 455 nm. Data presented were means ± standard error (bar) from three to four replicates of measurement. The activity was measured at 65°C. Note both transgenic line #4 and wild type (WT) had minimal activities.Back to article page