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Table 1 Effect of manner of ethanol addition on esterificationa

From: Two-step synthesis of fatty acid ethyl ester from soybean oil catalyzed by Yarrowia lipolytica lipase

   

Mean esterification ± SD, %

 

Addition method

Ethanol:fatty acid

molar ratio

Time, hours

Treatment

Control

Rate of increase, %

Three-step

2:1

3

60 ± 0.643

49.8 ± 0.493

20.5

Five-step

2:1

5

76.7 ± 0.794

51.5 ± 0.5

48.9

Ten-step

2:1

24

81.6 ± 0.6

54.1 ± 1.49

50.8

  1. aControl reaction used one-step addition of ethanol. Reaction system comprised 2.82 g of fatty acid, 580 × 2 μl of ethanol added in various ways, 0.33 g of immobilized lipase at 30°C and spun at 190 rpm. SD, standard deviation.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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