The role of acetyl xylan esterase in the solubilization of xylan and enzymatic hydrolysis of wheat straw and giant reed

Table 1 Carbohydrates released from pretreated wheat straw and giant reed by xylan-acting enzymes after 24 h hydrolysis in 50 mM sodium citrate buffer at pH 5.0 and 45°C

Substrate	Enzyme preparation	Xylose	Xylobiose	Xylotriose	Xylotetraose	Total
Wheat straw	XYL	1.6	7.2	1.0	bdl	9.8
	XYL+ARA	1.6	8.0	1.2	bdl	10.8
	XYL+AXE	2.2	9.1	bdl	bdl	11.3
Giant reed	XYL	1.7	7.5	2.3	1.7	13.2
	XYL+ARA	1.6	7.3	2.3	1.8	13.0
	XYL+AXE	3.1	10.6	bdl	bdl	13.7

The results are expressed as % of xylan (as xylose) in wheat straw or giant reed. The values of xylobiose, xylotriose and xylotetraose contained both acetylated and non-acetylated xylo-oligosaccharides. ARA: α-L-arabinofuranosidase; AXE: acetyl xylan esterase; bdl: below detection limit; XYL: xylanase.

ISSN: 2731-3654