Figure 2

Gating procedure performed in the dot plot combining fluorescence emission by FL2 and FL4 channels. The boxes represent the five clusters used during the gating of an environmental sample stained with the BODIPY 505/515 (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) solvatochromic dye.