Figure 5

Cells from the P4 gate internalized BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza- s -indacene) in lipid bodies. Cells were stained and sorted directly onto microscope slides after the sorting procedure. BODIPY dots could be observed with the FL1 channel (B, D), and in the merged differential interference contrast (DIC) system + BODIPY fluorescence (A, C). We clearly detected that BODIPY effectively stained lipid bodies in this strain (DIC in grey, BODIPY in green; scale bar = 5 μm).