Skip to main content

Table 1 Characteristics of the bacterial and yeast strains and plasmids used in this study

From: Direct ethanol production from cellulosic materials using a diploid strain of Saccharomyces cerevisiaewith optimized cellulase expression

Strains or plasmids

Relevant features

Reference

Bacterial strain

  

E. coli Novablue

endA1 hsdR17(r K12 -m K12 +) supE44 thi-I gyrA96 relA1 lac recA1/F'[proAB+ lacIq ZΔM15::Tn10(Tetr)]

Novagen

S. cerevisiae yeast strains

  

MT8-1

MATa ade his3 leu2 trp1 ura3

[17]

NBRC1440ΔHUWL

MATα his3 leu2 trp1 ura3

[13]

MT8-1/IBEC

MATa ade leu2, Single copy Integration of β-glucosidase, endoglucanase and cellobiohydrolase gene

[8]

MT8-1/cocδBEC3

MATa ade leu2, Cocktail δ-Integration of β-glucosidase, endoglucanase and cellobiohydrolase gene

[8]

MT8-1/cocδBEC3/LEU2

MATa ade, Cocktail δ-Integration of β-glucosidase, endoglucanase and cellobiohydrolase gene

This study

1440/cocδBEC3

MATα leu2, Cocktail δ-Integration of β-glucosidase, endoglucanase and cellobiohydrolase gene

This study

MNII/cocδBEC3

MATa/α, Cocktail δ-Integration of β-glucosidase, endoglucanase and cellobiohydrolase gene

This study

Plasmids

  

pδW-PGAGBGL

TRP1, Expression of β-glucosidase by δ-integration

[8]

pδU-PGAGBGL

URA3, Expression of β-glucosidase by δ-integration

[8]

pδH-PGAGBGL

HIS3, Expression of β-glucosidase by δ-integration

[8]

pδW-PGAGEG

TRP1, Expression endoglucanase by δ-integration

[8]

pδU-PGAGEG

URA3, Expression endoglucanase by δ-integration

[8]

pδH-PGAGEG

HIS3, Expression endoglucanase by δ-integration

[8]

pδW-PGAGCBH

TRP1, Expression cellobiohydrolase by δ-integration

[8]

pδU-PGAGCBH

URA3, Expression cellobiohydrolase by δ-integration

[8]

pδH-PGAGCBH

HIS3, Expression cellobiohydrolase by δ-integration

[8]

pRS405

LEU2, no expression

Stratagene