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Table 1 Characteristics of the bacterial and yeast strains and plasmids used in this study

From: Direct ethanol production from cellulosic materials using a diploid strain of Saccharomyces cerevisiaewith optimized cellulase expression

Strains or plasmids Relevant features Reference
Bacterial strain   
E. coli Novablue endA1 hsdR17(r K12 -m K12 +) supE44 thi-I gyrA96 relA1 lac recA1/F'[proAB+ lacIq ZΔM15::Tn10(Tetr)] Novagen
S. cerevisiae yeast strains   
MT8-1 MATa ade his3 leu2 trp1 ura3 [17]
NBRC1440ΔHUWL MATα his3 leu2 trp1 ura3 [13]
MT8-1/IBEC MATa ade leu2, Single copy Integration of β-glucosidase, endoglucanase and cellobiohydrolase gene [8]
MT8-1/cocδBEC3 MATa ade leu2, Cocktail δ-Integration of β-glucosidase, endoglucanase and cellobiohydrolase gene [8]
MT8-1/cocδBEC3/LEU2 MATa ade, Cocktail δ-Integration of β-glucosidase, endoglucanase and cellobiohydrolase gene This study
1440/cocδBEC3 MATα leu2, Cocktail δ-Integration of β-glucosidase, endoglucanase and cellobiohydrolase gene This study
MNII/cocδBEC3 MATa/α, Cocktail δ-Integration of β-glucosidase, endoglucanase and cellobiohydrolase gene This study
Plasmids   
pδW-PGAGBGL TRP1, Expression of β-glucosidase by δ-integration [8]
pδU-PGAGBGL URA3, Expression of β-glucosidase by δ-integration [8]
pδH-PGAGBGL HIS3, Expression of β-glucosidase by δ-integration [8]
pδW-PGAGEG TRP1, Expression endoglucanase by δ-integration [8]
pδU-PGAGEG URA3, Expression endoglucanase by δ-integration [8]
pδH-PGAGEG HIS3, Expression endoglucanase by δ-integration [8]
pδW-PGAGCBH TRP1, Expression cellobiohydrolase by δ-integration [8]
pδU-PGAGCBH URA3, Expression cellobiohydrolase by δ-integration [8]
pδH-PGAGCBH HIS3, Expression cellobiohydrolase by δ-integration [8]
pRS405 LEU2, no expression Stratagene