|
Bacterial strain
| | |
|
E. coli Novablue
|
endA1 hsdR17(r
K12
-m
K12
+) supE44 thi-I gyrA96 relA1 lac recA1/F'[proAB+ lacIq ZΔM15::Tn10(Tetr)]
|
Novagen
|
|
S. cerevisiae
yeast strains
| | |
|
MT8-1
|
MATa ade his3 leu2 trp1 ura3
|
[17]
|
|
NBRC1440ΔHUWL
|
MATα his3 leu2 trp1 ura3
|
[13]
|
|
MT8-1/IBEC
|
MATa ade leu2, Single copy Integration of β-glucosidase, endoglucanase and cellobiohydrolase gene
|
[8]
|
|
MT8-1/cocδBEC3
|
MATa ade leu2, Cocktail δ-Integration of β-glucosidase, endoglucanase and cellobiohydrolase gene
|
[8]
|
|
MT8-1/cocδBEC3/LEU2
|
MATa ade, Cocktail δ-Integration of β-glucosidase, endoglucanase and cellobiohydrolase gene
|
This study
|
|
1440/cocδBEC3
|
MATα leu2, Cocktail δ-Integration of β-glucosidase, endoglucanase and cellobiohydrolase gene
|
This study
|
|
MNII/cocδBEC3
|
MATa/α, Cocktail δ-Integration of β-glucosidase, endoglucanase and cellobiohydrolase gene
|
This study
|
|
Plasmids
| | |
|
pδW-PGAGBGL
|
TRP1, Expression of β-glucosidase by δ-integration
|
[8]
|
|
pδU-PGAGBGL
|
URA3, Expression of β-glucosidase by δ-integration
|
[8]
|
|
pδH-PGAGBGL
|
HIS3, Expression of β-glucosidase by δ-integration
|
[8]
|
|
pδW-PGAGEG
|
TRP1, Expression endoglucanase by δ-integration
|
[8]
|
|
pδU-PGAGEG
|
URA3, Expression endoglucanase by δ-integration
|
[8]
|
|
pδH-PGAGEG
|
HIS3, Expression endoglucanase by δ-integration
|
[8]
|
|
pδW-PGAGCBH
|
TRP1, Expression cellobiohydrolase by δ-integration
|
[8]
|
|
pδU-PGAGCBH
|
URA3, Expression cellobiohydrolase by δ-integration
|
[8]
|
|
pδH-PGAGCBH
|
HIS3, Expression cellobiohydrolase by δ-integration
|
[8]
|
|
pRS405
|
LEU2, no expression
|
Stratagene
|
