Strains or plasmids | Relevant features | Reference |
---|---|---|
Bacterial strain | ||
E. coli Novablue | endA1 hsdR17(r K12 -m K12 +) supE44 thi-I gyrA96 relA1 lac recA1/F'[proAB+ lacIq ZΔM15::Tn10(Tetr)] | Novagen |
S. cerevisiae yeast strains | ||
MT8-1 | MATa ade his3 leu2 trp1 ura3 | [17] |
NBRC1440ΔHUWL | MATα his3 leu2 trp1 ura3 | [13] |
MT8-1/IBEC | MATa ade leu2, Single copy Integration of β-glucosidase, endoglucanase and cellobiohydrolase gene | [8] |
MT8-1/cocδBEC3 | MATa ade leu2, Cocktail δ-Integration of β-glucosidase, endoglucanase and cellobiohydrolase gene | [8] |
MT8-1/cocδBEC3/LEU2 | MATa ade, Cocktail δ-Integration of β-glucosidase, endoglucanase and cellobiohydrolase gene | This study |
1440/cocδBEC3 | MATα leu2, Cocktail δ-Integration of β-glucosidase, endoglucanase and cellobiohydrolase gene | This study |
MNII/cocδBEC3 | MATa/α, Cocktail δ-Integration of β-glucosidase, endoglucanase and cellobiohydrolase gene | This study |
Plasmids | ||
pδW-PGAGBGL | TRP1, Expression of β-glucosidase by δ-integration | [8] |
pδU-PGAGBGL | URA3, Expression of β-glucosidase by δ-integration | [8] |
pδH-PGAGBGL | HIS3, Expression of β-glucosidase by δ-integration | [8] |
pδW-PGAGEG | TRP1, Expression endoglucanase by δ-integration | [8] |
pδU-PGAGEG | URA3, Expression endoglucanase by δ-integration | [8] |
pδH-PGAGEG | HIS3, Expression endoglucanase by δ-integration | [8] |
pδW-PGAGCBH | TRP1, Expression cellobiohydrolase by δ-integration | [8] |
pδU-PGAGCBH | URA3, Expression cellobiohydrolase by δ-integration | [8] |
pδH-PGAGCBH | HIS3, Expression cellobiohydrolase by δ-integration | [8] |
pRS405 | LEU2, no expression | Stratagene |