Strain | Cellulase expression | Initial PASC concentration, g/l | Maximum ethanol production, g/l | Maximum ethanol reaching time, hours | Ethanol yield of the theoretical yield from initial cellulose, %1 | Time and effort | Reference |
---|---|---|---|---|---|---|---|
S. cerevisiae 2 | Cell surface display | 10 | 2.9 | 40 | 57 | Triple yeast transformation | [3] |
S. cerevisiae | Secretion | 10 | 1.0 | 192 | 20 | Single yeast transformation | [2] |
S. cerevisiae and E. coli3 | Minicellulosome constructed by E. coli produced cellulases | 10 | 3.5 | 48 | 69 | Single yeast transformation, cellulase production by E. coli, and immobilization | [4] |
S. cerevisiae | Minicellulosome | 10 | 1.8 | 70 | 35 | Double yeast transformation | [5] |
S. cerevisiae | Cell surface display in optimum ratio | 20 | 3.1 | 72 | 30 | Triple yeast transformation | This study |
S. cerevisiae | Cell surface display in optimum ratio with diploidization | 20 | 7.6 | 72 | 75 | Triple yeast transformation and diploidization | This study |