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Table 3 Comparison of ethanol productivity from PASC for several cellulase-expression systems in S. cerevisiae

From: Direct ethanol production from cellulosic materials using a diploid strain of Saccharomyces cerevisiaewith optimized cellulase expression

Strain Cellulase expression Initial PASC concentration, g/l Maximum ethanol production, g/l Maximum ethanol reaching time, hours Ethanol yield of the theoretical yield from initial cellulose, %1 Time and effort Reference
S. cerevisiae 2 Cell surface display 10 2.9 40 57 Triple yeast transformation [3]
S. cerevisiae Secretion 10 1.0 192 20 Single yeast transformation [2]
S. cerevisiae and E. coli3 Minicellulosome constructed by E. coli produced cellulases 10 3.5 48 69 Single yeast transformation, cellulase production by E. coli, and immobilization [4]
S. cerevisiae Minicellulosome 10 1.8 70 35 Double yeast transformation [5]
S. cerevisiae Cell surface display in optimum ratio 20 3.1 72 30 Triple yeast transformation This study
S. cerevisiae Cell surface display in optimum ratio with diploidization 20 7.6 72 75 Triple yeast transformation and diploidization This study
  1. 1Calculated from each result.
  2. 2Saccharomyces cerevisiae.
  3. 3Escherichia coli.